This document is a compilation of the training materials used for the course entitled “Enhanced TB Microscopy Training of Trainers.” The course was held on July 25 to 29, 2016, at the Cebu TB Reference Laboratory in Cebu City, Philippines, and is part of the activities under the National TB Program (NTP) Laboratory Training Decentralization Strategy. This training of trainers (TOT) was planned, organized, and implemented jointly by SIAPS/PH, the National TB Reference Laboratory (NTRL), and the Department of Health (DOH) Regional Office for Central Visayas. The materials for this course were designed so that they can be used in the original or modified version in future TOTs for TB microscopy or for other NTP laboratory training. Trainers from the NTP will benefit from these materials as they will find them useful when they plan and conduct future trainings.