This guide aims to help individuals, organizations, and governments develop, establish, and maintain effective multidisciplinary and multisectoral coalitions to combat AMR. This document provides guidance on building and strengthening coalitions to combat AMR through five key elements. It primarily focuses on the creation and establishment of new coalitions; however, these elements are relevant throughout the lifespan of a coalition, and revisiting each periodically may be important as coalitions grow and evolve, expand in scope or wane and need to be revitalized. In addition, the elements of coalition building may not necessarily occur in strict sequential order. Depending on the context and existing systems in place, coalitions may be able to use situational analyses that have already been conducted or utilize an existing active network. In each setting, it is important to understand the needs of the coalition and focus on the elements of this guide that align best with those needs.